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Berkala Penelitian Hayati
Published by Perhimpunan Biologi Indonesia Cabang Jawa Timur
ISSN : 08526834     EISSN : 2337389X     DOI : https://doi.org/10.23869/bphjbr
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology
Berkala Penelitian Hayati is a half yearly international peer reviewed, an open access life science journal. The journal was published by The East Java Biological Society and formerly used the Indonesian language. The first edition of this journal is Vol 1 No 1 in June 1995. It was accredited by Ministry of Culture and Education. It continues recorded by Zoological Record by Thomson Reuters Clarivate Analytics since 2011. Since April 2012, the journal was changed into English. This journal is indexed by DOAJ, Crossref, Google Scholar, Academia.edu, and EBSCO Host. This journal publishes original research, applied, review article, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. This journal publishes original research, applied, review articles, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. The journal scopes include, but are not limited to, the following topic areas including botany, zoology, ecology, microbiology, physiology, nanobiology, coastal biology, hydrobiology, neurobiology, genetics, developmental biology, biochemistry and molecular biology, biophysics, and life science.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 7 Documents
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Search results for , issue "Vol 1 No 1 (1995): June 1995" : 7 Documents clear
PENGARUH MANIPULASI MEDIA TERHADAP KANDUNGAN ALKALOID VINKRISTINA KALUS DAUN Catharanthus roseus(L.) G.Don Y. Sri Wulan Manuhara
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 1 No 1 (1995): June 1995
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (425.823 KB) | DOI: 10.23869/163

Abstract

The effect of the manipulation of the media on the vincristine alkaloid content in the callus of Catharanthus roseus (L.) G.Don were studied. This work was done as an effort for gaining the vincristine alkaloid through tissue culture which was expected to obtain a larger amount of the alkaloid. Tissue culture of C. roseus was initiated from leaf explants on growth medium (MS supplemented with 1 mg/l 2,4-D and 1 mg/l BAP). after seven weeks incubation, with only subculturing on the same medium, the proliferating calli were subculture on a production medium (MS supplemented with 1mg/l IAA and 1 mg/l BAP) which were containing different additional concentration of sucrose, BAP, tryptophan, and concentration of 50% basic medium of MS from the standard. Eleven weeks-old calli were harvested from each treatment and dried for chemical analysed by thin layer chromatography on silica gel GF 254 using chloroform-aceton-triethylamine as mobile phase. Rf value and uv spectra were used to identify vincristine, and concentration of vincristine alkaloid was determine by preparative thin layer chromatography with methanol solvent and measured by uv-vis spectrophotometer at 233 nm. The maximum content of vincristine alkaloid was obtained from callus, which was grown on the MS standard with an addition of 40 g/l sucrose or 4 mg/l BAP or and also 50% of the MS standard medium. Tryptophan addition a precursor could not induce the alkaloid vincristine forming.
ISOLASI DAN KARAKTERISASI MUTAN sal4 DI RAGI Saccharomyces cereviceae Ni Nyoman Tri Puspaningsih
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 1 No 1 (1995): June 1995
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (385.012 KB) | DOI: 10.23869/164

Abstract

Recently, genetics manipulation in yeast Saccharomyces cereviceae have much been done. It because yeast can be used as a host cell alternative in the foreign protein expression, therefore information about fidelity from yeast should be studied. Preliminary study showed that SAL 4 gene has assumed to has a role in translation fidelity control and/or termination factor. To study the gene function, mutation in yeast BSC483/1a has been done by Ethylmethane sulphonate. Mutants wished are mutated at sal 4 locus and have characteristic of both allosuppressor and omnipotent suppressor. Phenotype of allosuppresor mutants were indicated by white colour consistency in YPD and Y8 medium, temperature sensitivity, paromomycin sensitivity and growth rate. Quantitatively, effectiveness as omnipotent suppressor has been done by using gene fusion between PGK and B-galaktosidase. The result showed that BSC483/1a strain could be mutated by Ethylmethane sulphonate 1% and produced eight allosuppressor mutants. Two of them (Number 8 and 10) have characteristic of temperature sensitivity, and the two others (Number 1 and 13) were mutated at sal 4 gene locus. Characterize of sal 4 mutants (1 and 13) didn't show temperature sensitive and have growth rate relatively more slowly than the wild type. Mutant (number 13) could suppress nonsense mutation (realthrough) at termination codon UAG with B-galaktosidase activity as amount 2.70 unit/ml.
ANALISIS KANDUNGAN KUMAN ENTERIK PADA AIR KEMIH Agus Supriyanto; Noer Moehammadi
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 1 No 1 (1995): June 1995
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (231.648 KB) | DOI: 10.23869/165

Abstract

This research was conducted in order to know the total late count (TPC) and what kind of enteric bacteria were isolated from urine specimens. Samples were taken from clinical laboratory, and was examinated in the section of microbiology department of Biology, FMIPAUniversitas Airlangga Surabaya. Thirty five samples were examinated. Method of detection of enteric bacteria, carried out in several steps including enrichment, isolation and identification. Identification step, was done using test of fisiology, motility and morphology with the Gram staining. The result showed, that the number of bacteria in urine specimens was 10 x 103 CFU per ml to 296 x 107 CFU per ml and consist of Enterobacter sp., Pseudomonas sp., Klebsiella sp., Serratia sp., Poteus morgagni, Proteus mirabilis, Aeromonas sp. and Escherichia coli.
PENGARUH KADAR AIR TANAH TERHADAP BIOMASSA CACING TANAH Pontoscolex corethrurus Alfiah Hayati
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 1 No 1 (1995): June 1995
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (213.428 KB) | DOI: 10.23869/166

Abstract

This research was done in order to study of effect the soil humidity on earthworm Pontoscolex corethrurus biomass. 80 earthworms were placed in plastic pot (12.5 cm in diameter and 10 cm on deep) contained soil media (four individuals in each pot). Four treatment of humidity (45 %, 5 %, 55 % and 60%) with five replication was carried out up to six weeks. At the end of experiment animals were weighting. The difference between pre and post experimental body weigh was calculated, F test was used to test biomass differences among the four conditions of humidity. The result shown that the optimum biomass was in 50% soil humidity in the soil media.
OPTIMALISASI KONDISI AMOBILISASI GLUKOMINASE MENGGUNAKAN PADATAN PENDUKUNG BENTONIT BENTUK ION UNTUK KONVERSI PATI MENJADI GULA Afaf Baktir; Sri Sumarsih; Hamami
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 1 No 1 (1995): June 1995
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (337.807 KB) | DOI: 10.23869/167

Abstract

Bentonit is a clay available in a lot of quantity and very chief. It can be activated to cation or anion exchanger like resin. His research was done in order to search a treatment and to get optimum condition for binding the glucoamylase to the bentonit, and to observe the ability of immobilized glucoamylase obtained in hydrolyzing amylum. It was done three ways to activate the bentonit, to obtain three kinds of bentonit, those are 'active bentonit' (adsorbs enzyme physically) and 'cation exchanger bentonit' consist B- NH4+, and B-H+ (bind enzyme ionically). The bentonit was contacted to glucoamylase in various condition to search the optimum condition (pH, concentration, temperature, and contact duration time). Immobilized glucomynase obtained was tested it's binding strengthness and the ability of hydrolyze amylum to glucose. The conclusion of this research were, (1) bentonit can be used as a supporting material for immobilizing glucomynase in the form of B-H+ cation exchanger, (2) the optimum condition of bentonit and glucomylase binding was in pH = 5.5 and at 25oC, contact duration time was 30 minutes, (3) immobilizes glucomylase column (height of 1 cm and diameter of 2.5 cm) had conversion ability at the level of 19.88%.
STRUKTUR MOLEKUL SENYAWA ASAM 3,4 SECO BRIONONAT DALAM KULIT BATANG TANAMAN KECAPI KERA Sandoricum emarginatum Hiern Pratiwi Pudjiastuti
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 1 No 1 (1995): June 1995
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (351.443 KB) | DOI: 10.23869/168

Abstract

Kecapi kera (Sandoricum emarginatum Hiern) is a plant grow in Sumatera, Indonesia bong the familia Meliaceae. There are two species of Sandoricum in Indonesia, Sandoricum koetjape and Sandoricum emarginatum. The S.koetjape plant has been investigated and contains some chemical compounds and there are two of which have a cytotoxic activity to the cell culture P 388 but Sandoricum emarginatum not yet investigated. The aim of this research was to determine the molecular structure compound of stem bark of Kecapi kera which soluble in chloroform. The chemical compound in stem bark of Kecapi kera was extracted with petroleum ether, chloroform, and methanol, respectively. Chloroform fraction was separated and isolated with column chromatography, n-hexane-ethyl acetate as mobile phase and silica gel as stationary phase. Molecular structure determination was carried out using spectroscopy method such IR, NMR, and MS. As the result a white crystal, 3,4-seco bryo-nonat could be isolated. The crystal has a needle like form and had melting point between 226-228oC.
PENGARUH PEMETIKAN PUCUK DAN SAMPING TUNAS TANAMAN TEMBAKAU (Nicotiana tabacum L.) TERHADAP KERUSAKAN DAUN Hani Sudarmanto
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 1 No 1 (1995): June 1995
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (412.629 KB) | DOI: 10.23869/169

Abstract

Tanaman tembakau merupakan semak setahun yang telah lama dikenal dan dibudidayakan di Indonesia. Menurut penggunaannya, dibedakan tembakau sigaret/virginia, tembakau cerutu/vorstenlands, dan tembakau rajangan. Tembakau cerutu ditanam pada musim penghujan, sedangkan tembakau sigaret dan rajangan ditanam pada musim kemarau (Utami dkk, 1993). Dalam budidaya tembakau, kualitas daun lebih diutamakan daripada kuantitasnya. Kualitas tembakau dapat dilihat dari warna, aroma, rasa, dan keutuhan daunnya. Aroma dan rasa daun tembakau ditentukan oleh kandungan kimianya, di antaranya kadar nikotin. keutuhan daun ditentukan oleh ada-tidaknya serangan serangga hama. kadar nikotin daun dapat ditingkatkan dengan pemetikan pucuk dan tunas samping tanaman tembakau. Hasil penelitian Fahrurozi (1983) sebagaimana termuat dalam Berita Biologi 2 (1), 1983, menunjukkan bahwa pemetikan pucuk dan tunas samping tanaman tembakau dapat meningkatkan kadar nikotin rata-rata 3.935% dibandingkan tanaman tembakau yang tidak dipetik pucuk dan tunas sampingnya (1.657%). Yang menjadi permasalahan adalah apakah pemetikan pucuk dan tunas samping tanaman tembakau tersebut juga dapat menurunkan tingkat kerusakan daun yang diakibatkan oleh serangan serangga hama. Hal itu dapat diakibatkan dengan fungsi nikotin (alkaloid) sebagai senjata kimiawi khususnya insect repellents (Ehrlich and Raven,1967). Asumsi yang digunakan adalah peningkatan kadar nikotin dapat menurunkan toleransi serangga hama terhadap kadar nikotin tinggi, sehingga tingkat serangan serangga hama menjadi berkurang

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